murine l5178y r lymphoma cells  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Eggplant ( Solanum spp.) Fruits Dietary Polyphenols Upregulate the Expression of Glucose Transporter Protein in Palmitate-Induced Diabetic Cell Line C2C12

doi: 10.3390/ijms26167762

Figure Lengend Snippet: Shows the image of the cell population of the C2C12 cell plates at Magnification: 100×; Scale Bar: 100 μm. KEY : ( A ): C2C12: Normal cells without the myotubes; ( B ): Differentiated cells Positive Control (healthy matured cells) with myotubes; ( C ): Negative control induced with 0.75 mM Palmitate (diabetic cells); ( D ): Induced with 0.75 mM Palmitate + 0.1 µM Metformin (diabetic cells treated with metformin); ( E ): Induced with 0.75 mM Palmitate + PG (diabetic cells treated with 150 µg/mL Solanum kumba ); ( F ): Induced with 0.75 mM Palmitate + PGW (diabetic cells treated with 150 µg/mL Solanum aethiopicum ); ( G ): Healthy matured cells with 150 µg/mL PG ( Solanum kumba ); ( H ): Healthy matured cells with 150 µg/mL PGW ( Solanum aethiopicum ).

Article Snippet: Protease tablets were purchased from Roche (South San Francisco, CA, USA), while reverse and forward primers were gotten from Integrated DNA Technologies (Coralville, IA, USA) as well as the reference gene β-actin, Murine C2C12 skeletal muscle cells (CRL 1722) were obtained from the American Type Culture Collection (Manassas, VA, USA) and cell culture kits for cellular antioxidants were supplied by Sigma Aldrich (St. Louis, MO, USA).

Techniques: Positive Control, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Eggplant ( Solanum spp.) Fruits Dietary Polyphenols Upregulate the Expression of Glucose Transporter Protein in Palmitate-Induced Diabetic Cell Line C2C12

doi: 10.3390/ijms26167762

Figure Lengend Snippet: Intracellular effect of eggplant extracts on the antioxidant enzymes glutathione peroxidase ( A ), catalase ( B ), superoxide dismutase ( C ), and glucose oxidase ( D ) activities in C2C12 cells. Values represent mean ± standard deviation (n = 3). Bars with the same letter are not significant ( p < 0.05). KEY: from the left to the right on the bar chart (Control): is normal cells (Pal): is the diabetic cells without treatment, (Pal + Mef): is diabetic cells with metformin, (Pal + PG): diabetic cells + Solanum kumba , (Pal + PGW): diabetic cells + Solanum aethiopicum , (PG): normal cells + Solanum kumba , (PGW): normal cells + Solanum aethiopicum .

Article Snippet: Protease tablets were purchased from Roche (South San Francisco, CA, USA), while reverse and forward primers were gotten from Integrated DNA Technologies (Coralville, IA, USA) as well as the reference gene β-actin, Murine C2C12 skeletal muscle cells (CRL 1722) were obtained from the American Type Culture Collection (Manassas, VA, USA) and cell culture kits for cellular antioxidants were supplied by Sigma Aldrich (St. Louis, MO, USA).

Techniques: Standard Deviation, Control

Journal: International Journal of Molecular Sciences

Article Title: Eggplant ( Solanum spp.) Fruits Dietary Polyphenols Upregulate the Expression of Glucose Transporter Protein in Palmitate-Induced Diabetic Cell Line C2C12

doi: 10.3390/ijms26167762

Figure Lengend Snippet: The genetic expression with RT-qPCR on GLUT4 , NRF-1 , and MEF-2A in C2C12 cells.

Article Snippet: Protease tablets were purchased from Roche (South San Francisco, CA, USA), while reverse and forward primers were gotten from Integrated DNA Technologies (Coralville, IA, USA) as well as the reference gene β-actin, Murine C2C12 skeletal muscle cells (CRL 1722) were obtained from the American Type Culture Collection (Manassas, VA, USA) and cell culture kits for cellular antioxidants were supplied by Sigma Aldrich (St. Louis, MO, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Slit1 -a MET target gene in the embryonic limbs, prevents premature differentiation during mammalian myogenesis

doi: 10.1101/2025.06.22.660860

Figure Lengend Snippet: A-C.Representative immunoblots using lysates of C2C12 cells, showing levels of phosphorylated (P)-MET tyrosine 1234/1235 and total (T)-MET (A), SLIT1, P-p44/42 and T-p44/42 (8), and M-CAD, P-Akt and T-Akt (C), in response to HGF-mediated stimulation, inhibition by SU11274, sequential treatment with SU11274 and HGF, and treatment with respective solvents/vehicles in which the pharmacological reagents were dissolved. A’-C”. Bar graphs showing densitometric quantitation of P-MET 1234/1235 (A’), SLIT1 (B’), P-p44/42 (B”), M-CAD (C’) and P-Akt (C”) using the expression data from immunoblots (A-C). D-D”. Representative western blots and corresponding bar graphs showing densitometric values of SLIT1 (D and D’) and P-p44/42 (D and D”) levels upon treatment of C2C12 cells with HGF, U0126, U0126+HGF, and with respective solvents of these reagents. E-E”. Representative immunoblots and corresponding densitometry data graphs showing levels of M-CAD (E and E’) and P-Akt (E and E”) in C2C12 cells treated with HGF, Wortmannin (WO), WO+HGF and their respective solvents. Densitometry data in the bar graphs represent treatment-responsive protein expression normalized initially to the reference gene and then to protein levels in the respective vehicle/solvent controls. Graphical data are represented as means ±SD and significance was tested using one-way ANOVA. 37

Article Snippet: C2C12 mouse myoblast cells (American Type Culture Collection-ATCC; Cat# CRL-1722) were cultured and maintained in complete medium [CM = DMEM (Thermo Fisher Scientific; GIBCO, Cat# 11995065) + 10% fetal bovine serum (FBS, Thermo Fisher Scientific; GIBCO, Cat# 10270106) + 1% Penicillin-Streptomycin (Thermo Fisher Scientific; GIBCO, Cat# 15240062)], according to ATCC recommendations.

Techniques: Western Blot, Inhibition, Quantitation Assay, Expressing, Solvent

Journal: bioRxiv

Article Title: Slit1 -a MET target gene in the embryonic limbs, prevents premature differentiation during mammalian myogenesis

doi: 10.1101/2025.06.22.660860

Figure Lengend Snippet: Silencing Slit1 in murine myoblasts leads to pre-mature differentiation. A-A”’. Control siRNA transfected C2C12 mouse myoblasts show myotube formation by Day (D) 5 of differentiation protocol. B-B”’. siRNA-mediated downregulation of Slit1 in C2C12 cells leads to the formation of myofiber like structures, highlighted by black arrows (B’), quite premtaurely i.e. on second day (D2) of differentiation. C. Representative immunoblots showing levels of SLIT1 and ≥-ACTIN, at DO, D2, D5, and D7 of myoblast differentiation, in cell lysates of C2C12 cells transfected with control or $/it1 siRNA. D-E. Bar graphs representing densitometric analyses of SLIT1 expression at DO and D2 (D), and at DO, D5, and D7 (E) of differentiation, in control versus Slit1 siRNA transfected C2C12 lysates as represented in the corresponding immunoblots (C). F-1. Representative western blots (F) and their densitometric quantitation (G-J), depicting expression of Myosin (F-H) and Cleaved Caspase 3 (CC-3) (F, 1-J) at indicated day (D) of myogenic differentiation in lysates from C2C12 cells transfected with control or Slit1 siRNA. For densitometric quantification, SLIT1, Myosin and CC-3 expression was normalized to corresponding ≥-ACTIN levels at each differentiation day for both (control and $/it1) siRNA transfections. Expression of gene of interest, normalized to reference gene values at indicated days of differentiation, were in turn normalised to similarly normalized DO values of the respective siRNA transfection. The graphical data are represented as means +SD and were subjected to multiple unpaired T-tests. ns=non-significant.

Article Snippet: C2C12 mouse myoblast cells (American Type Culture Collection-ATCC; Cat# CRL-1722) were cultured and maintained in complete medium [CM = DMEM (Thermo Fisher Scientific; GIBCO, Cat# 11995065) + 10% fetal bovine serum (FBS, Thermo Fisher Scientific; GIBCO, Cat# 10270106) + 1% Penicillin-Streptomycin (Thermo Fisher Scientific; GIBCO, Cat# 15240062)], according to ATCC recommendations.

Techniques: Control, Transfection, Western Blot, Expressing, Quantitation Assay